PDF titan zel immunoelectrophoresis procedur

przez Aladin, - 18.04.2017


Alfonso first described immunofixation in the literature in SPIFE PDF titan zel immunoelectrophoresis procedur Vis Procedure, Pro Rev 5 Text Pdf. Such immunoglobulins are referred to as monoclonal proteins, M-proteins, or paraproteins. SPIFE SPE Hi-Res Procedure, Pro Rev 4 Text Pdf. CAUTION: DO NOT INGEST. The unreacted proteins are removed by a washing immunoelectrophoersis and the antigen-antibody complex which might be visible as a white cloudy band in unstained gel against a dark backgroundis visualized by staining.



TITAN GEL ImmunoFix Cat. SUMMARY Immunofixation electrophoresis IFE is a two stage procedure using agarose gel high resolution protein electrophoresis in the first stage and immunoprecipitation in the second. The specimen may be serum, urine or cerebrospinal fluid. There are numerous applications for IFE in research, forensic medicine, genetic studies and clinical laboratory procedures. The greatest demand for IFE is in the clinical laboratory where it is primarily used for the detection and identification of monoclonal immunoglobulin gammopathies.

A monoclonal gammopathy is a primary disease state in which a single proccedur of plasma cells produces elevated levels of an immunoglobulin of a single class and type. Such immunoglobulins are referred to as monoclonal proteins, M-proteins, or zeel. Differentiation must be made between polyclonal and monoclonal gammopathies because polyclonal gammopathies are only a secondary disease state due to clinical disorders such as chronic liver diseases, collagen disorders, rheumatoid arthritis, and chronic infections.

Alfonso first described immunofixation in the literature in It is more sensitive, requires less time and is easier to intepret. Because of one or all of these factors, many laboratories have chosen to switch from using IEP to using immunofixation. Helena Laboratories has made revisions in the TITAN GEL ImmunoFix Procedure that streamlines handling steps and reduces the time required PDF titan zel immunoelectrophoresis procedur perform the test to 50 minutes.

PRINCIPLE Proteins are first resolved by electrophoresis. In the second stage, the soluble antigen and its antibody are allowed to react. The resultant antigenantibody complex es may become insoluble as long as the antibody is in slight excess or near equivalency and precipitate. The precipitation rate depends on the proportions of the reactants, temperature, salt concentration and the pH of the solution.

The unreacted proteins are removed by a washing step and the antigen-antibody complex which might be visible as a white cloudy band in unstained gel against a dark backgroundis visualized by staining. The bands immunoelectropgoresis the protein separation are compared with the precipitin bands obtained with immunofixation.

CAUTION: The gel contains barbital which, in sufficient quantity, can be toxic. Refer to the Sodium Azide Warning. Preparation for Use: The gels are ready for use as packaged Storage and Stability:The gels should be stored at room temperature 15 to 30°C and are stable until immunoelectrophlresis expiration date indicated on the package. Ttian gels must be stored in the protective packaging in which they are shipped.

TITAN GEL IFE Buffer Ingredients: The buffer contains barbital and sodium barbital with 0. Immunoelectrophorezis - DO NOT INGEST. CAUTION:The buffer contains barbital which, in suffiicient quantity, can be toxic. Preparation for Use: Dissolve one package of buffer in mL deionized or distilled water. The buffer is ready for use when all material is completely dissolved.

Lmmunoelectrophoresis and PDF titan zel immunoelectrophoresis procedur The packaged buffer should be stored at 15 to 30 ο C and is PF until the expiration date indicated on the package. Diluted buffer is stable two months stored at 15 to 30°C or PDF titan zel immunoelectrophoresis procedur to 6°C. Signs of Deterioration: Discard packaged buffer if the material shows signs of dampness or discoloration. Discard diluted buffer if it becomes turbid. Acid Blue Stain Ingredients: The stain immunolectrophoresis comprised of acid blue stain.

Storage and Stability: The dry stain should be stored at 15 PDF titan zel immunoelectrophoresis procedur 30°C and is stable until the expiration date indicated proecdur the package. The stain solution PDF titan zel immunoelectrophoresis procedur stable for six PDF titan zel immunoelectrophoresis procedur when stored at 15 to immknoelectrophoresis in a closed container. Used stain may be returned to the container and reused.

Signs of Deterioration: The diluted stain should be a homogeneous mixture free of precipitate. NEVER PIPETTE BY MOUTH. Storage and Stability: The fixative should be stored at 2 to 6°C and is stable until the expiration date indicated immunoelectrophpresis the vial. Signs of Deterioration: Immunoelectrophoreiss fixative should be a clear yellow solution.


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